HMBS Kit ELISA

N° du produit ABIN5655222

Détail du produit HMBS Kit ELISA

Antigène: HMBS (Hydroxymethylbilane Synthase (HMBS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hydroxymethylbilane Synthase (HMBS). No significant cross-reactivity or interference between Hydroxymethylbilane Synthase (HMBS) and analogues was observed.

Sensibilité: 0.26 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxymethylbilane Synthase (HMBS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydroxymethylbilane Synthase (HMBS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxymethylbilane Synthase (HMBS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxymethylbilane Synthase (HMBS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hydroxymethylbilane Synthase (HMBS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hydroxymethylbilane Synthase (HMBS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HMBS (Hydroxymethylbilane Synthase (HMBS))

Autre désignation: Hydroxymethylbilane Synthase (HMBS Produits)

Synonymes: PBG-D Kit ELISA, PBGD Kit ELISA, PORC Kit ELISA, UPS Kit ELISA, URO-S Kit ELISA, hemC Kit ELISA, pbg-d Kit ELISA, pbgd Kit ELISA, ups Kit ELISA, SPAC806.01 Kit ELISA, DDBDRAFT_0186148 Kit ELISA, DDBDRAFT_0231417 Kit ELISA, DDB_0186148 Kit ELISA, DDB_0231417 Kit ELISA, Hmbs Kit ELISA, NV50236 Kit ELISA, T25658 Kit ELISA, Ups Kit ELISA, Uros1 Kit ELISA, hmbs Kit ELISA, zgc:64128 Kit ELISA, hmbsl Kit ELISA, id:ibd5004 Kit ELISA, im:7140060 Kit ELISA, zgc:110690 Kit ELISA, hydroxymethylbilane synthase Kit ELISA, hydroxymethylbilane synthase S homeolog Kit ELISA, hydroxymethylbilane synthase L homeolog Kit ELISA, hydroxymethylbilane synthase (predicted) Kit ELISA, Hydroxymethylbilane synthase Kit ELISA, hydroxymethylbilane synthase a Kit ELISA, hydroxymethylbilane synthase, b Kit ELISA, HMBS Kit ELISA, Hmbs Kit ELISA, hmbs Kit ELISA, hmbs.2.S Kit ELISA, hmbs.L Kit ELISA, CNC02250 Kit ELISA, hem3 Kit ELISA, hemC Kit ELISA, Trad_0335 Kit ELISA, Plabr_2100 Kit ELISA, Sgly_3113 Kit ELISA, SMLT_RS19660 Kit ELISA, PMI_RS16580 Kit ELISA, hmbsa Kit ELISA, hmbsb Kit ELISA

Sujet:

Gene Name: Hydroxymethylbilane Synthase

Gene Aliases: PBG-D, PBGD, UPS, Hydroxymethylbilane Synthase, Uroporphyrinogen I Synthase, Porphobilinogen deaminase, Pre-uroporphyrinogen synthase

ID gène: 3145

UniProt: P08397