HPRT1 Kit ELISA

N° du produit ABIN5655227

Détail du produit HPRT1 Kit ELISA

Antigène: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). No significant cross-reactivity or interference between Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxanthine Phosphoribosyltransferase 1 (HPRT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Autre désignation: Hypoxanthine Phosphoribosyltransferase 1 (HPRT1 Produits)

Synonymes: HGPRT Kit ELISA, HPRT Kit ELISA, C81579 Kit ELISA, HPGRT Kit ELISA, Hprt1 Kit ELISA, Hgprtase Kit ELISA, Hprt Kit ELISA, id:ibd1344 Kit ELISA, id:ibd5108 Kit ELISA, wu:fc10g09 Kit ELISA, zgc:56221 Kit ELISA, zgc:86608 Kit ELISA, hgprt Kit ELISA, hprt Kit ELISA, prtfdc1 Kit ELISA, hprt1 Kit ELISA, hprt1l Kit ELISA, zgc:55561 Kit ELISA, zgc:86771 Kit ELISA, hypoxanthine phosphoribosyltransferase 1 Kit ELISA, hypoxanthine guanine phosphoribosyl transferase Kit ELISA, hypoxanthine phosphoribosyltransferase 1 L homeolog Kit ELISA, phosphoribosyl transferase domain containing 1 Kit ELISA, HPRT1 Kit ELISA, Hprt Kit ELISA, Hprt1 Kit ELISA, hprt1 Kit ELISA, hprt1.L Kit ELISA, prtfdc1 Kit ELISA

Sujet:

Gene Name: Hypoxanthine Phosphoribosyltransferase 1

Gene Aliases: HGPRT, HGPRTase, HPRT, Lesch-Nyhan Syndrome, Hypoxanthine-guanine phosphoribosyltransferase

ID gène: 3251

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process