EPAS1 Kit ELISA

N° du produit ABIN5655241

Détail du produit EPAS1 Kit ELISA

Antigène: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 2 Alpha (HIF2a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 2 Alpha (HIF2a) and analogues was observed.

Sensibilité: 27 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 2 Alpha (HIF2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 2 Alpha (HIF2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 2 Alpha (HIF2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hypoxia Inducible Factor 2 Alpha (HIF2a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hypoxia Inducible Factor 2 Alpha (HIF2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Autre désignation: Hypoxia Inducible Factor 2 Alpha (EPAS1 Produits)

Synonymes: EPAS1 Kit ELISA, ECYT4 Kit ELISA, HIF2A Kit ELISA, HLF Kit ELISA, MOP2 Kit ELISA, PASD2 Kit ELISA, bHLHe73 Kit ELISA, HIF-2alpha Kit ELISA, HRF Kit ELISA, Hif2a Kit ELISA, bhlhe73 Kit ELISA, ecyt4 Kit ELISA, hif2 Kit ELISA, hif2a Kit ELISA, hif2alpha Kit ELISA, hlf Kit ELISA, mop2 Kit ELISA, pasd2 Kit ELISA, EPAS Kit ELISA, EPAS-1 Kit ELISA, endothelial PAS domain protein 1 Kit ELISA, endothelial PAS domain protein 1 L homeolog Kit ELISA, EPAS1 Kit ELISA, epas1 Kit ELISA, Epas1 Kit ELISA, epas1.L Kit ELISA

Sujet:

Gene Name: Hypoxia Inducible Factor 2 Alpha

Gene Aliases: EPAS1, HLF, MOP2, PASD2, BHLHE73, Endothelial PAS Domain 1, HIF-1 Alpha-Like Factor, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73

ID gène: 29452

UniProt: Q9JHS1

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, L'effet Warburg