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IgG2a Kit ELISA

Reactivité: Souris Colorimetric Sandwich ELISA 7.81 ng/mL - 500 ng/mL Plasma, Serum
N° du produit ABIN5655311
  • Antigène Tous les produits IgG2a
    IgG2a
    Reactivité
    • 4
    • 3
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    7.81 ng/mL - 500 ng/mL
    Seuil minimal de détection
    7.81 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G2a (IgG2a). No significant cross-reactivity or interference between Immunoglobulin G2a (IgG2a) and analogues was observed.
    Sensibilité
    3.8 ng/mL
    Featured
    Discover our best selling IgG2a Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G2a (IgG2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G2a (IgG2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin G2a (IgG2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G2a (IgG2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G2a (IgG2a) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G2a (IgG2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Tous les produits IgG2a
    IgG2a
    Autre désignation
    Immunoglobulin G2a (IgG2a Produits)
    Synonymes
    gamma-2a immunoglobulin heavy chain Kit ELISA, IgG-2a Kit ELISA
    Classe de substances
    Antibody
    Sujet

    Gene Name: Immunoglobulin G2a

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