ICOSLG Kit ELISA

N° du produit ABIN5655335

Détail du produit ICOSLG Kit ELISA

Antigène: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Inducible T-Cell Co Stimulator Ligand (ICOSLG). No significant cross-reactivity or interference between Inducible T-Cell Co Stimulator Ligand (ICOSLG) and analogues was observed.

Sensibilité: 0.062 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inducible T-Cell Co Stimulator Ligand (ICOSLG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inducible T-Cell Co Stimulator Ligand (ICOSLG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inducible T-Cell Co Stimulator Ligand (ICOSLG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inducible T-Cell Co Stimulator Ligand (ICOSLG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inducible T-Cell Co Stimulator Ligand (ICOSLG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inducible T-Cell Co Stimulator Ligand (ICOSLG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Autre désignation: Inducible T-Cell Co Stimulator Ligand (ICOSLG Produits)

Synonymes: ICOSLG Kit ELISA, AU044799 Kit ELISA, B7-H2 Kit ELISA, B7RP-1 Kit ELISA, B7h Kit ELISA, BG071784 Kit ELISA, GI50 Kit ELISA, GL50 Kit ELISA, GL50-B Kit ELISA, ICOS-L Kit ELISA, Icoslg Kit ELISA, LICOS Kit ELISA, Ly115l Kit ELISA, mKIAA0653 Kit ELISA, B7H2 Kit ELISA, B7RP1 Kit ELISA, CD275 Kit ELISA, ICOSL Kit ELISA, Icosl Kit ELISA, RGD1562791 Kit ELISA, inducible T-cell costimulator ligand Kit ELISA, icos ligand Kit ELISA, inducible T-cell co-stimulator ligand Kit ELISA, ICOSLG Kit ELISA, Icosl Kit ELISA, Icoslg Kit ELISA

Sujet:

Gene Name: Inducible T-Cell Co Stimulator Ligand

Gene Aliases: CD275, B7-H2, B7H2, B7RP-1, B7RP1, GL50, ICOS-L, ICOSL, LICOS, B7-like protein Gl50, B7-related protein 1

ID gène: 102723996

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation