IDE Kit ELISA

N° du produit ABIN5655389

Détail du produit IDE Kit ELISA

Antigène: IDE (Insulin-Degrading Enzyme (IDE))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Insulin Degrading Enzyme (IDE). No significant cross-reactivity or interference between Insulin Degrading Enzyme (IDE) and analogues was observed.

Sensibilité: 0.125 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Degrading Enzyme (IDE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Degrading Enzyme (IDE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Degrading Enzyme (IDE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Degrading Enzyme (IDE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Degrading Enzyme (IDE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Degrading Enzyme (IDE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IDE (Insulin-Degrading Enzyme (IDE))

Autre désignation: Insulin Degrading Enzyme (IDE Produits)

Synonymes: zgc:162603 Kit ELISA, IDE Kit ELISA, 1300012G03Rik Kit ELISA, 4833415K22Rik Kit ELISA, AA675336 Kit ELISA, AI507533 Kit ELISA, INSDEGM Kit ELISA, INSULYSIN Kit ELISA, insulin degrading enzyme Kit ELISA, insulin-degrading enzyme Kit ELISA, metallopeptidase Kit ELISA, IDE Kit ELISA, ide Kit ELISA, LOC591315 Kit ELISA, CC1G_01115 Kit ELISA, Bm1_26615 Kit ELISA, PTRG_10875 Kit ELISA, SJAG_01430 Kit ELISA, EBI_21700 Kit ELISA, VDBG_06702 Kit ELISA, LOAG_08082 Kit ELISA, PGTG_04982 Kit ELISA, Tsp_00424 Kit ELISA, Ide Kit ELISA

Sujet:

Gene Name: Insulin Degrading Enzyme

Gene Aliases: Insulysin, Insulin Protease, Abeta-degrading protease, Insulinase

Pathways: SARS-CoV-2 Protein Interactome