IGF2BP3 Kit ELISA

N° du produit ABIN5655415

Détail du produit IGF2BP3 Kit ELISA

Antigène: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). No significant cross-reactivity or interference between Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) and analogues was observed.

Sensibilité: 0.064 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Autre désignation: Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3 Produits)

Synonymes: CT98 Kit ELISA, IMP-3 Kit ELISA, IMP3 Kit ELISA, KOC1 Kit ELISA, VICKZ3 Kit ELISA, Ab2-255 Kit ELISA, RGD1306512 Kit ELISA, imp3 Kit ELISA, koc1 Kit ELISA, vera Kit ELISA, imp-3 Kit ELISA, vg1rbp Kit ELISA, vickz3 Kit ELISA, 2610101N11Rik Kit ELISA, AA522010 Kit ELISA, AL022933 Kit ELISA, AU045931 Kit ELISA, Koc13 Kit ELISA, Neilsen Kit ELISA, mimp3 Kit ELISA, dvr1rbp Kit ELISA, wu:fb79c12 Kit ELISA, wu:fe06h09 Kit ELISA, zVg1RBP Kit ELISA, B3 Kit ELISA, IGF2 mRNA-binding protein 3-B Kit ELISA, IMP Kit ELISA, IMP-3-B Kit ELISA, Vg1 RBP-B Kit ELISA, Vg1-RBP Kit ELISA, igf2bp3 Kit ELISA, igf2bp3-a Kit ELISA, igf2bp3-b Kit ELISA, IGF2 mRNA-binding protein 3-A Kit ELISA, IMP-3-A Kit ELISA, Vg1 RBP-A Kit ELISA, vera-A Kit ELISA, insulin like growth factor 2 mRNA binding protein 3 Kit ELISA, insulin-like growth factor 2 mRNA binding protein 3 Kit ELISA, insulin like growth factor 2 mRNA binding protein 3 S homeolog Kit ELISA, insulin like growth factor 2 mRNA binding protein 3 L homeolog Kit ELISA, IGF2BP3 Kit ELISA, Igf2bp3 Kit ELISA, igf2bp3 Kit ELISA, igf2bp3.S Kit ELISA, igf2bp3.L Kit ELISA

Sujet:

Gene Name: Insulin Like Growth Factor 2 mRNA Binding Protein 3

Gene Aliases: MP-3, IMP3, KOC1, VICKZ3, CT98, IGF II mRNA Binding Protein 3, Cancer/Testis Antigen 98, KH domain-containing protein overexpressed in cancer

ID gène: 10643

UniProt: O00425