CD47 Kit ELISA

N° du produit ABIN5655499

Détail du produit CD47 Kit ELISA

Antigène: CD47

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Integrin Associated Protein (IAP). No significant cross-reactivity or interference between Integrin Associated Protein (IAP) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Associated Protein (IAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Associated Protein (IAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Associated Protein (IAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Associated Protein (IAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Associated Protein (IAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Associated Protein (IAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CD47

Autre désignation: Integrin Associated Protein (CD47 Produits)

Synonymes: IAP Kit ELISA, MER6 Kit ELISA, OA3 Kit ELISA, 9130415E20Rik Kit ELISA, AA407862 Kit ELISA, AI848868 Kit ELISA, AW108519 Kit ELISA, B430305P08Rik Kit ELISA, Itgp Kit ELISA, CD47/IAP Kit ELISA, alkaline phosphatase, intestinal Kit ELISA, CD47 molecule Kit ELISA, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) Kit ELISA, Cd47 molecule Kit ELISA, ALPI Kit ELISA, CD47 Kit ELISA, Cd47 Kit ELISA

Sujet:

Gene Name: Integrin Associated Protein

Gene Aliases: CD47, MER6, OA3, Antigen Identified By Monoclonal Antibody 1D8, Antigenic Surface Determinant Protein OA3, Rh-Related Antigen

ID gène: 961

UniProt: Q08722