Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

ICAM4 Kit ELISA

ICAM4 Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5655535
  • Antigène Voir toutes ICAM4 Kits ELISA
    ICAM4 (Intercellular Adhesion Molecule 4 (ICAM4))
    Reactivité
    • 2
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 4 (ICAM4). No significant cross-reactivity or interference between Intercellular Adhesion Molecule 4 (ICAM4) and analogues was observed.
    Sensibilité
    0.063 ng/mL
    Featured
    Discover our best selling ICAM4 Kit ELISA
    Top Product
    Discover our top product ICAM4 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 4 (ICAM4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 4 (ICAM4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Intercellular Adhesion Molecule 4 (ICAM4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Intercellular Adhesion Molecule 4 (ICAM4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 4 (ICAM4) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 4 (ICAM4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes ICAM4 Kits ELISA
    ICAM4 (Intercellular Adhesion Molecule 4 (ICAM4))
    Autre désignation
    Intercellular Adhesion Molecule 4 (ICAM4 Produits)
    Synonymes
    CD242 Kit ELISA, LW Kit ELISA, 1810015M19Rik Kit ELISA, Cd242 Kit ELISA, ICAM-4 Kit ELISA, intercellular adhesion molecule 4 (Landsteiner-Wiener blood group) Kit ELISA, intercellular adhesion molecule 4, Landsteiner-Wiener blood group Kit ELISA, intercellular adhesion molecule 4 Kit ELISA, ICAM4 Kit ELISA, Icam4 Kit ELISA
    Sujet

    Gene Name: Intercellular Adhesion Molecule 4

    Gene Aliases: CD242, LW, Landsteiner-Weiner Blood System, Landsteiner-Wiener blood group glycoprotein

    ID gène
    3386
    UniProt
    Q14773
Vous êtes ici:
Support technique