IDH1 Kit CLIA

N° du produit ABIN5656119

Détail du produit IDH1 Kit CLIA

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 ng/mL - 500 ng/mL

Seuil minimal de détection: 7.81 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 1, Soluble (IDH1). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 1, Soluble (IDH1) and analogues was observed.

Sensibilité: 0.28 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Isocitrate Dehydrogenase 1, Soluble (IDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Isocitrate Dehydrogenase 1, Soluble (IDH1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isocitrate Dehydrogenase 1, Soluble (IDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Autre désignation: Isocitrate Dehydrogenase 1, Soluble (IDH1 Produits)

Synonymes: IDCD Kit CLIA, IDH Kit CLIA, IDP Kit CLIA, IDPC Kit CLIA, PICD Kit CLIA, NADP-CICDH Kit CLIA, AI314845 Kit CLIA, AI788952 Kit CLIA, E030024J03Rik Kit CLIA, Id-1 Kit CLIA, Idh-1 Kit CLIA, Idpc Kit CLIA, cb876 Kit CLIA, fm90e09 Kit CLIA, im:7143416 Kit CLIA, wu:fm90e09 Kit CLIA, F23E12.180 Kit CLIA, F23E12_180 Kit CLIA, IDH-I Kit CLIA, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 Kit CLIA, isocitrate dehydrogenase 1 Kit CLIA, isocitrate dehydrogenase I Kit CLIA, isocitrate dehydrogenase (NADP(+)) 1, cytosolic Kit CLIA, isocitrate dehydrogenase 1 (NADP+), soluble Kit CLIA, isocitrate dehydrogenase 1 (NADP+) L homeolog Kit CLIA, isocitrate dehydrogenase 1 Kit CLIA, IDH1 Kit CLIA, Idh1 Kit CLIA, idh1.L Kit CLIA, idh1 Kit CLIA

Sujet:

Gene Name: Isocitrate Dehydrogenase 1, Soluble

Gene Aliases: PICD, IDP, Oxalosuccinate decarboxylase, NADP(+)-specific ICDH, Cytosolic NADP-isocitrate dehydrogenase

ID gène: 3417

UniProt: O75874

Pathways: L'effet Warburg