IARS Kit ELISA

N° du produit ABIN5656124

Détail du produit IARS Kit ELISA

Antigène: IARS (Isoleucyl-tRNA Synthetase (IARS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Isoleucyl tRNA Synthetase (IARS). No significant cross-reactivity or interference between Isoleucyl tRNA Synthetase (IARS) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isoleucyl tRNA Synthetase (IARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isoleucyl tRNA Synthetase (IARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isoleucyl tRNA Synthetase (IARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isoleucyl tRNA Synthetase (IARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isoleucyl tRNA Synthetase (IARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isoleucyl tRNA Synthetase (IARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IARS (Isoleucyl-tRNA Synthetase (IARS))

Autre désignation: Isoleucyl tRNA Synthetase (IARS Produits)

Synonymes: fi46h05 Kit ELISA, zgc:63790 Kit ELISA, wu:fi46h05 Kit ELISA, K19E20.18 Kit ELISA, K19E20_18 Kit ELISA, ovule abortion 2 Kit ELISA, ECK0027 Kit ELISA, ilvS Kit ELISA, JW0024 Kit ELISA, An08g06770 Kit ELISA, AO090012000505 Kit ELISA, 2510016L12Rik Kit ELISA, AI327140 Kit ELISA, AU044614 Kit ELISA, E430001P04Rik Kit ELISA, ILRS Kit ELISA, Iarsl Kit ELISA, IARS1 Kit ELISA, ILERS Kit ELISA, IRS Kit ELISA, PRO0785 Kit ELISA, isoleucyl-tRNA synthetase Kit ELISA, tRNA synthetase class I (I, L, M and V) family protein Kit ELISA, isoleucine--tRNA ligase Kit ELISA, isoleucyl-tRNA synthetase, cytoplasmic Kit ELISA, isoleucine-tRNA synthetase Kit ELISA, iars Kit ELISA, IARS Kit ELISA, OVA2 Kit ELISA, ileS Kit ELISA, MBAR_RS19160 Kit ELISA, Rmag_0340 Kit ELISA, ANI_1_964074 Kit ELISA, CHLREDRAFT_106327 Kit ELISA, EDI_049880 Kit ELISA, OE_RS08520 Kit ELISA, AOR_1_1912194 Kit ELISA, Iars Kit ELISA

Sujet:

Gene Name: Isoleucyl tRNA Synthetase

Gene Aliases: IARS1, ILRS, OJ, IleRS, IRS, Isoleucine tRNA Ligase 1,Cytoplasmic

ID gène: 3376

UniProt: P41252