JAM2 Kit ELISA

N° du produit ABIN5656138

Détail du produit JAM2 Kit ELISA

Antigène: JAM2 (Junctional Adhesion Molecule 2 (JAM2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Junctional Adhesion Molecule 2 (JAM2). No significant cross-reactivity or interference between Junctional Adhesion Molecule 2 (JAM2) and analogues was observed.

Sensibilité: 12.7 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Junctional Adhesion Molecule 2 (JAM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Junctional Adhesion Molecule 2 (JAM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Junctional Adhesion Molecule 2 (JAM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Junctional Adhesion Molecule 2 (JAM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Junctional Adhesion Molecule 2 (JAM2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Junctional Adhesion Molecule 2 (JAM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: JAM2 (Junctional Adhesion Molecule 2 (JAM2))

Autre désignation: Junctional Adhesion Molecule 2 (JAM2 Produits)

Synonymes: JAM2 Kit ELISA, cd322 Kit ELISA, jam-b Kit ELISA, jamb Kit ELISA, pro245 Kit ELISA, ve-jam Kit ELISA, vejam Kit ELISA, jam2 Kit ELISA, sb:cb1002 Kit ELISA, im:7155552 Kit ELISA, sc:d0813 Kit ELISA, si:ch211-160b11.5 Kit ELISA, LOC100227766 Kit ELISA, 1110002N23Rik Kit ELISA, 2410030G21Rik Kit ELISA, 2410167M24Rik Kit ELISA, JAM-2 Kit ELISA, JAM-B Kit ELISA, Jcam2 Kit ELISA, VE-JAM Kit ELISA, C21orf43 Kit ELISA, CD322 Kit ELISA, JAMB Kit ELISA, PRO245 Kit ELISA, VEJAM Kit ELISA, junctional adhesion molecule 2 Kit ELISA, junctional adhesion molecule 2a Kit ELISA, junctional adhesion molecule 2b Kit ELISA, junction adhesion molecule 2 Kit ELISA, JAM2 Kit ELISA, jam2 Kit ELISA, jam2a Kit ELISA, jam2b Kit ELISA, Jam2 Kit ELISA

Sujet:

Gene Name: Junctional Adhesion Molecule 2

Gene Aliases: CD322, JAM-B, JAMB, VE-JAM, VEJAM, Junctional adhesion molecule B, Vascular endothelial junction-associated molecule

ID gène: 58494

UniProt: P57087