KEAP1 Kit ELISA

N° du produit ABIN5656175

Détail du produit KEAP1 Kit ELISA

Antigène: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Kelch Like ECH Associated Protein 1 (KEAP1). No significant cross-reactivity or interference between Kelch Like ECH Associated Protein 1 (KEAP1) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1 (KEAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kelch Like ECH Associated Protein 1 (KEAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kelch Like ECH Associated Protein 1 (KEAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kelch Like ECH Associated Protein 1 (KEAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Autre désignation: Kelch Like ECH Associated Protein 1 (KEAP1 Produits)

Synonymes: INrf2 Kit ELISA, KLHL19 Kit ELISA, INRF2 Kit ELISA, mKIAA0132 Kit ELISA, Inrf2 Kit ELISA, fb36f11 Kit ELISA, keap1 Kit ELISA, wu:fb36f11 Kit ELISA, KEAP1 Kit ELISA, klhl19 Kit ELISA, zgc:171654 Kit ELISA, kelch like ECH associated protein 1 Kit ELISA, kelch-like ECH-associated protein 1 Kit ELISA, Kelch-like ECH-associated protein 1 Kit ELISA, kelch-like ECH-associated protein 1a Kit ELISA, kelch like ECH associated protein 1 L homeolog Kit ELISA, kelch-like ECH-associated protein 1b Kit ELISA, KEAP1 Kit ELISA, Keap1 Kit ELISA, keap1a Kit ELISA, keap1 Kit ELISA, keap1.L Kit ELISA, keap1b Kit ELISA

Sujet:

Gene Name: Kelch Like ECH Associated Protein 1

Gene Aliases: INrf2, KLHL19, Cytosolic inhibitor of Nrf2, Kelch-like protein 19

ID gène: 9817

UniProt: Q14145

Pathways: Maintenance of Protein Location