Keratin 10 Kit ELISA

N° du produit ABIN5656179

Détail du produit Keratin 10 Kit ELISA

Antigène: Keratin 10 (KRT10)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Keratin 10 (KRT10). No significant cross-reactivity or interference between Keratin 10 (KRT10) and analogues was observed.

Sensibilité: 12.2 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 10 (KRT10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 10 (KRT10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 10 (KRT10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 10 (KRT10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 10 (KRT10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 10 (KRT10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Keratin 10 (KRT10)

Autre désignation: Keratin 10 (KRT10 Produits)

Synonymes: BCIE Kit ELISA, BIE Kit ELISA, CK10 Kit ELISA, EHK Kit ELISA, K10 Kit ELISA, KPP Kit ELISA, D130054E02Rik Kit ELISA, K1C1 Kit ELISA, Krt-1.10 Kit ELISA, Krt1-10 Kit ELISA, Ka10 Kit ELISA, Ker10 Kit ELISA, KRT10 Kit ELISA, KRT25 Kit ELISA, KRT27 Kit ELISA, KRT28 Kit ELISA, keratin 10 Kit ELISA, keratin 10, type I S homeolog Kit ELISA, keratin, type I cytoskeletal 25 Kit ELISA, KRT10 Kit ELISA, Krt10 Kit ELISA, krt10.S Kit ELISA, LOC101085587 Kit ELISA

Sujet:

Gene Name: Keratin 10

Gene Aliases: K10, CK10, KPP, Cytokeratin 10, Epidermolytic Hyperkeratosis, Keratosis Palmaris Et Plantaris, Keratin, type I cytoskeletal 10

ID gène: 3858

UniProt: P13645