LACTB Kit ELISA

N° du produit ABIN5656269

Détail du produit LACTB Kit ELISA

Antigène: LACTB (Lactamase, beta (LACTB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lactamase Beta (LACTb). No significant cross-reactivity or interference between Lactamase Beta (LACTb) and analogues was observed.

Sensibilité: 0.122 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactamase Beta (LACTb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactamase Beta (LACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactamase Beta (LACTb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactamase Beta (LACTb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactamase Beta (LACTb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactamase Beta (LACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LACTB (Lactamase, beta (LACTB))

Autre désignation: Lactamase Beta (LACTB Produits)

Synonymes: BA2507 Kit ELISA, PSPTO2834 Kit ELISA, PSPTO3594 Kit ELISA, G24 Kit ELISA, MRPL56 Kit ELISA, LACT-1 Kit ELISA, Lact1 Kit ELISA, Mrpl56 Kit ELISA, zgc:110419 Kit ELISA, beta-lactamase Kit ELISA, class C beta-lactamase AmpC Kit ELISA, metal-dependent hydrolase Kit ELISA, lactamase beta Kit ELISA, Beta-lactamase Kit ELISA, lactamase, beta Kit ELISA, metallo-beta-lactamase domain containing 2 Kit ELISA, Beta lactamase Kit ELISA, bla1 Kit ELISA, ampC Kit ELISA, PSPTO_2834 Kit ELISA, GSU1378 Kit ELISA, MCA2220 Kit ELISA, CJE0344 Kit ELISA, LACTB Kit ELISA, Rmar_1357 Kit ELISA, MGYG_07395 Kit ELISA, ML5_3452 Kit ELISA, bla Kit ELISA, Lactb Kit ELISA, lactb Kit ELISA, MBLAC2 Kit ELISA, blaKPC-2 Kit ELISA

Sujet:

Gene Name: Lactamase Beta

Gene Aliases: G24, MRPL56, Mitochondrial Ribosomal Protein L56, Serine beta-lactamase-like protein LACTB, mitochondrial

ID gène: 114294

UniProt: P83111