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LDHB Kit ELISA

LDHB Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5656276
  • Antigène Voir toutes LDHB Kits ELISA
    LDHB (Lactate Dehydrogenase B (LDHB))
    Reactivité
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Lactate Dehydrogenase B (LDHB). No significant cross-reactivity or interference between Lactate Dehydrogenase B (LDHB) and analogues was observed.
    Sensibilité
    0.133 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactate Dehydrogenase B (LDHB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactate Dehydrogenase B (LDHB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactate Dehydrogenase B (LDHB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactate Dehydrogenase B (LDHB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactate Dehydrogenase B (LDHB) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactate Dehydrogenase B (LDHB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes LDHB Kits ELISA
    LDHB (Lactate Dehydrogenase B (LDHB))
    Autre désignation
    Lactate Dehydrogenase B (LDHB Produits)
    Synonymes
    LDH-B Kit ELISA, LDH-H Kit ELISA, LDHBD Kit ELISA, TRG-5 Kit ELISA, AI790582 Kit ELISA, H-Ldh Kit ELISA, Ldh-2 Kit ELISA, Ldh2 Kit ELISA, ldh-h Kit ELISA, ldh2 Kit ELISA, ldhb Kit ELISA, ldhba Kit ELISA, trg-5 Kit ELISA, LDHB Kit ELISA, LDH-B-A Kit ELISA, wu:fa16d07 Kit ELISA, wu:fa20c10 Kit ELISA, wu:fa96a08 Kit ELISA, LDH-B-B Kit ELISA, zgc:92882 Kit ELISA, lactate dehydrogenase B Kit ELISA, lactate dehydrogenase B S homeolog Kit ELISA, lactate dehydrogenase Ba Kit ELISA, lactate dehydrogenase Bb Kit ELISA, LDHB Kit ELISA, Ldhb Kit ELISA, ldhb.S Kit ELISA, ldhb Kit ELISA, ldhba Kit ELISA, ldhbb Kit ELISA
    Sujet

    Gene Name: Lactate Dehydrogenase B

    Gene Aliases: LDH-B, LDH-H, Renal carcinoma antigen NY-REN-46

    ID gène
    3945
    UniProt
    P07195
    Pathways
    L'effet Warburg
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