LMAN2 Kit ELISA

N° du produit ABIN5656344

Détail du produit LMAN2 Kit ELISA

Antigène: LMAN2 (Lectin, Mannose-Binding 2 (LMAN2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lectin, Mannose Binding 2 (LMAN2). No significant cross-reactivity or interference between Lectin, Mannose Binding 2 (LMAN2) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lectin, Mannose Binding 2 (LMAN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lectin, Mannose Binding 2 (LMAN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lectin, Mannose Binding 2 (LMAN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lectin, Mannose Binding 2 (LMAN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lectin, Mannose Binding 2 (LMAN2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lectin, Mannose Binding 2 (LMAN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LMAN2 (Lectin, Mannose-Binding 2 (LMAN2))

Autre désignation: Lectin, Mannose Binding 2 (LMAN2 Produits)

Synonymes: Lman2 Kit ELISA, wu:fb16f04 Kit ELISA, zgc:158761 Kit ELISA, C5orf8 Kit ELISA, GP36B Kit ELISA, VIP36 Kit ELISA, 1110003H06Rik Kit ELISA, 1300009F09Rik Kit ELISA, AA408240 Kit ELISA, AL023023 Kit ELISA, AU040819 Kit ELISA, Vip36 Kit ELISA, lectin, mannose binding 2 Kit ELISA, lectin, mannose-binding 2 Kit ELISA, LMAN2 Kit ELISA, lman2 Kit ELISA, Lman2 Kit ELISA

Sujet:

Gene Name: Lectin, Mannose Binding 2

Gene Aliases: GP36B, VIP36, C5orf8, Vesicular integral-membrane protein VIP36, Glycoprotein GP36b, Vesicular integral-membrane protein 36

ID gène: 10960

UniProt: Q12907

Pathways: SARS-CoV-2 Protein Interactome