LEFTY1 Kit CLIA

N° du produit ABIN5656345

Détail du produit LEFTY1 Kit CLIA

Antigène: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1). No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.

Sensibilité: 5.31 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Autre désignation: Left/Right Determination Factor 1 (LEFTY1 Produits)

Synonymes: atv Kit CLIA, cb73 Kit CLIA, antivin Kit CLIA, ik:tdsubc_2b12 Kit CLIA, xx:tdsubc_2b12 Kit CLIA, LEFTY1 Kit CLIA, AI450052 Kit CLIA, Ebaf Kit CLIA, Leftb Kit CLIA, Stra3 Kit CLIA, Tgfb4 Kit CLIA, lefty Kit CLIA, lefty-1 Kit CLIA, RGD1561867 Kit CLIA, LEFTB Kit CLIA, LEFTYB Kit CLIA, lefty1 Kit CLIA, signaling molecule lefty1 Kit CLIA, left-right determination factor 1 Kit CLIA, left right determination factor 1 Kit CLIA, lft1 Kit CLIA, lefty1 Kit CLIA, LOC699924 Kit CLIA, LEFTY1 Kit CLIA, LOC100408003 Kit CLIA, LOC100597378 Kit CLIA, Lefty1 Kit CLIA

Sujet:

Gene Name: Left/Right Determination Factor 1

Gene Aliases: LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B

ID gène: 10637

UniProt: O75610