LEFTY1 Kit ELISA

N° du produit ABIN5656346

Détail du produit LEFTY1 Kit ELISA

Antigène: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1). No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.

Sensibilité: 0.31 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Left/Right Determination Factor 1 (LEFTY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Left/Right Determination Factor 1 (LEFTY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Autre désignation: Left/Right Determination Factor 1 (LEFTY1 Produits)

Synonymes: atv Kit ELISA, cb73 Kit ELISA, antivin Kit ELISA, ik:tdsubc_2b12 Kit ELISA, xx:tdsubc_2b12 Kit ELISA, LEFTY1 Kit ELISA, AI450052 Kit ELISA, Ebaf Kit ELISA, Leftb Kit ELISA, Stra3 Kit ELISA, Tgfb4 Kit ELISA, lefty Kit ELISA, lefty-1 Kit ELISA, RGD1561867 Kit ELISA, LEFTB Kit ELISA, LEFTYB Kit ELISA, lefty1 Kit ELISA, signaling molecule lefty1 Kit ELISA, left-right determination factor 1 Kit ELISA, left right determination factor 1 Kit ELISA, lft1 Kit ELISA, lefty1 Kit ELISA, LOC699924 Kit ELISA, LEFTY1 Kit ELISA, LOC100408003 Kit ELISA, LOC100597378 Kit ELISA, Lefty1 Kit ELISA

Sujet:

Gene Name: Left/Right Determination Factor 1

Gene Aliases: LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B

ID gène: 10637

UniProt: O75610