LGMN Kit ELISA

N° du produit ABIN5656353

Détail du produit LGMN Kit ELISA

Antigène: LGMN (Legumain (LGMN))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Legumain (LGMN). No significant cross-reactivity or interference between Legumain (LGMN) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Legumain (LGMN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Legumain (LGMN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Legumain (LGMN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Legumain (LGMN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Legumain (LGMN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Legumain (LGMN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LGMN (Legumain (LGMN))

Autre désignation: Legumain (LGMN Produits)

Synonymes: AEP Kit ELISA, LGMN1 Kit ELISA, PRSC1 Kit ELISA, AI746452 Kit ELISA, AU022324 Kit ELISA, Prsc1 Kit ELISA, MGC64351 Kit ELISA, cb173 Kit ELISA, cb460 Kit ELISA, sb:cb173 Kit ELISA, zgc:76953 Kit ELISA, wu:fa98g05 Kit ELISA, wu:fj92g08 Kit ELISA, lgmn Kit ELISA, MGC88980 Kit ELISA, DELTA VACUOLAR PROCESSING ENZYME Kit ELISA, DELTAVPE Kit ELISA, delta vacuolar processing enzyme Kit ELISA, legumain Kit ELISA, legumain L homeolog Kit ELISA, delta vacuolar processing enzyme Kit ELISA, Legumain Kit ELISA, LGMN Kit ELISA, Lgmn Kit ELISA, lgmn.L Kit ELISA, lgmn Kit ELISA, DELTA-VPE Kit ELISA, MBOO_RS07785 Kit ELISA, Odosp_3582 Kit ELISA, LOC569153 Kit ELISA

Sujet:

Gene Name: Legumain

Gene Aliases: AEP, LGMN1, PRSC1, Protease,Cysteine 1, Asparaginyl endopeptidase

ID gène: 19141

UniProt: O89017

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades