LASP1 Kit ELISA

N° du produit ABIN5656420

Détail du produit LASP1 Kit ELISA

Antigène: LASP1 (LIM and SH3 Protein 1 (LASP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of LIM And SH3 Protein 1 (LASP1). No significant cross-reactivity or interference between LIM And SH3 Protein 1 (LASP1) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM And SH3 Protein 1 (LASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM And SH3 Protein 1 (LASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM And SH3 Protein 1 (LASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM And SH3 Protein 1 (LASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM And SH3 Protein 1 (LASP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM And SH3 Protein 1 (LASP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LASP1 (LIM and SH3 Protein 1 (LASP1))

Autre désignation: LIM And SH3 Protein 1 (LASP1 Produits)

Synonymes: lasp1 Kit ELISA, MGC53336 Kit ELISA, LASP1 Kit ELISA, MGC89667 Kit ELISA, Lasp-1 Kit ELISA, MLN50 Kit ELISA, AA408629 Kit ELISA, Def-4 Kit ELISA, SH3P6 Kit ELISA, Tg(Col1a1-lacZ)1Ngma Kit ELISA, fb92f05 Kit ELISA, wu:fb92f05 Kit ELISA, zgc:77542 Kit ELISA, lasp-1 Kit ELISA, mln50 Kit ELISA, LIM and SH3 protein 1 Kit ELISA, LIM and SH3 protein 1 L homeolog Kit ELISA, lasp1 Kit ELISA, lasp1.L Kit ELISA, LASP1 Kit ELISA, Lasp1 Kit ELISA

Sujet:

Gene Name: LIM And SH3 Protein 1

Gene Aliases: MLN50, Metastatic lymph node gene 50 protein

ID gène: 3927

UniProt: Q14847