Lipase A Kit ELISA

N° du produit ABIN5656426

Détail du produit Lipase A Kit ELISA

Antigène: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lipase A, Lysosomal Acid (LIPA). No significant cross-reactivity or interference between Lipase A, Lysosomal Acid (LIPA) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, Lysosomal Acid (LIPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, Lysosomal Acid (LIPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase A, Lysosomal Acid (LIPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase A, Lysosomal Acid (LIPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase A, Lysosomal Acid (LIPA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase A, Lysosomal Acid (LIPA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Autre désignation: Lipase A, Lysosomal Acid (LIPA Produits)

Synonymes: cesd Kit ELISA, lal Kit ELISA, LIPA Kit ELISA, CESD Kit ELISA, LAL Kit ELISA, Chole Kit ELISA, Chole2 Kit ELISA, Lip1 Kit ELISA, AA960673 Kit ELISA, Lal Kit ELISA, Lip-1 Kit ELISA, lipase A, lysosomal acid type Kit ELISA, lipase A, lysosomal acid, cholesterol esterase Kit ELISA, lipase A, lysosomal acid, cholesterol esterase L homeolog Kit ELISA, lysosomal acid lipase A Kit ELISA, LIPA Kit ELISA, lipa Kit ELISA, lipa.L Kit ELISA, Lipa Kit ELISA

Sujet:

Gene Name: Lipase A, Lysosomal Acid

Gene Aliases: LAL, CESD, Lipase A,Lysosomal Acid,Cholesterol Esterase(Wolman Disease), Acid Cholesteryl Ester Hydrolase, Lysosomal acid lipase/cholesteryl ester hydrolase

ID gène: 16889

UniProt: Q9Z0M5