LPAR1 Kit ELISA

N° du produit ABIN5656538

Détail du produit LPAR1 Kit ELISA

Antigène: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lysophosphatidic Acid Receptor 1 (LPAR1). No significant cross-reactivity or interference between Lysophosphatidic Acid Receptor 1 (LPAR1) and analogues was observed.

Sensibilité: 0.112 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidic Acid Receptor 1 (LPAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysophosphatidic Acid Receptor 1 (LPAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidic Acid Receptor 1 (LPAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysophosphatidic Acid Receptor 1 (LPAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysophosphatidic Acid Receptor 1 (LPAR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysophosphatidic Acid Receptor 1 (LPAR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Autre désignation: Lysophosphatidic Acid Receptor 1 (LPAR1 Produits)

Synonymes: EDG2 Kit ELISA, GPR26 Kit ELISA, Gpcr26 Kit ELISA, LPA1 Kit ELISA, Mrec1.3 Kit ELISA, VZG1 Kit ELISA, edg-2 Kit ELISA, rec.1.3 Kit ELISA, vzg-1 Kit ELISA, Edg2 Kit ELISA, EDG-2 Kit ELISA, 5031439C20 Kit ELISA, AI326300 Kit ELISA, Kdt2 Kit ELISA, lpA1 Kit ELISA, edg2 Kit ELISA, si:dz22b13.1 Kit ELISA, si:dz46m7.1 Kit ELISA, zgc:92157 Kit ELISA, LPA receptor 1-A Kit ELISA, LPA-1-A Kit ELISA, gpr26 Kit ELISA, lpa1 Kit ELISA, lpa1r Kit ELISA, lpa1r1 Kit ELISA, lpar1 Kit ELISA, lpar1-a Kit ELISA, lpar1-b Kit ELISA, vzg1 Kit ELISA, xLPA1-1 Kit ELISA, LPA receptor 1-B Kit ELISA, LPA-1-B Kit ELISA, edg2-A Kit ELISA, xLPA1-2 Kit ELISA, xlpa1R Kit ELISA, lysophosphatidic acid receptor 1 Kit ELISA, lysophosphatidic acid receptor 1 S homeolog Kit ELISA, lysophosphatidic acid receptor 1 L homeolog Kit ELISA, LPAR1 Kit ELISA, Lpar1 Kit ELISA, lpar1 Kit ELISA, lpar1.S Kit ELISA, lpar1.L Kit ELISA

Sujet:

Gene Name: Lysophosphatidic Acid Receptor 1

Gene Aliases: EDG2, Gpcr26, LPA1, Mrec1.3, edg-2, rec.1.3, vzg-1, Endothelial Differentiation,Lysophosphatidic Acid G-Protein-Coupled Receptor 2

Pathways: Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration