KARS Kit ELISA

N° du produit ABIN5656570

Détail du produit KARS Kit ELISA

Antigène: KARS (Lysyl-tRNA Synthetase (KARS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lysyl tRNA Synthetase (KARS). No significant cross-reactivity or interference between Lysyl tRNA Synthetase (KARS) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl tRNA Synthetase (KARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl tRNA Synthetase (KARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl tRNA Synthetase (KARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl tRNA Synthetase (KARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: KARS (Lysyl-tRNA Synthetase (KARS))

Autre désignation: Lysyl tRNA Synthetase (KARS Produits)

Synonymes: CG12141 Kit ELISA, Dmel\\CG12141 Kit ELISA, KRS Kit ELISA, LysRS Kit ELISA, cb530 Kit ELISA, wu:fa16h02 Kit ELISA, zgc:92483 Kit ELISA, kars Kit ELISA, MGC53345 Kit ELISA, kars2 Kit ELISA, krs Kit ELISA, krs-1 Kit ELISA, KARS Kit ELISA, Tb06.28P18.190 Kit ELISA, CMTRIB Kit ELISA, DFNB89 Kit ELISA, KARS1 Kit ELISA, KARS2 Kit ELISA, AA589550 Kit ELISA, AL024334 Kit ELISA, AL033315 Kit ELISA, AL033367 Kit ELISA, D8Ertd698e Kit ELISA, D8Wsu108e Kit ELISA, mKIAA0070 Kit ELISA, Lysyl-tRNA synthetase Kit ELISA, lysyl-tRNA synthetase Kit ELISA, lysyl-tRNA synthetase S homeolog Kit ELISA, lysyl-tRNA synthetase L homeolog Kit ELISA, LysRS Kit ELISA, kars Kit ELISA, kars.S Kit ELISA, kars.L Kit ELISA, Bm1_16500 Kit ELISA, lysS Kit ELISA, Taci_1118 Kit ELISA, Tpau_1737 Kit ELISA, Arch_0261 Kit ELISA, Trad_2960 Kit ELISA, Spirs_3699 Kit ELISA, KARS Kit ELISA, LOC100280510 Kit ELISA, Tb927.6.1510 Kit ELISA, Kars Kit ELISA

Sujet:

Gene Name: Lysyl tRNA Synthetase

Gene Aliases: KARS2, LysRS, Lysine tRNA Ligase

ID gène: 3735

UniProt: Q15046