MDH1 Kit ELISA

N° du produit ABIN5656628

Détail du produit MDH1 Kit ELISA

Antigène: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Malate Dehydrogenase 1 (MDH1). No significant cross-reactivity or interference between Malate Dehydrogenase 1 (MDH1) and analogues was observed.

Sensibilité: 0.072 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Malate Dehydrogenase 1 (MDH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Malate Dehydrogenase 1 (MDH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Malate Dehydrogenase 1 (MDH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Malate Dehydrogenase 1 (MDH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Malate Dehydrogenase 1 (MDH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Malate Dehydrogenase 1 (MDH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Autre désignation: Malate Dehydrogenase 1 (MDH1 Produits)

Synonymes: MDH-s Kit ELISA, MDHA Kit ELISA, MGC:1375 Kit ELISA, MOR2 Kit ELISA, ODS1 Kit ELISA, B230377B03Rik Kit ELISA, D17921 Kit ELISA, Mor-2 Kit ELISA, Mor2 Kit ELISA, MDL1 Kit ELISA, Mdhl Kit ELISA, MDH Kit ELISA, mdh-s Kit ELISA, mdha Kit ELISA, mor2 Kit ELISA, MDH-1 Kit ELISA, Mdh Kit ELISA, Mdh-1 Kit ELISA, Mdh2 Kit ELISA, MdhD Kit ELISA, cMdh Kit ELISA, sMdh Kit ELISA, malate dehydrogenase 1 Kit ELISA, malic enzyme 2 Kit ELISA, malate dehydrogenase 1, NAD (soluble) Kit ELISA, malate dehydrogenase 1 S homeolog Kit ELISA, malate dehydrogenase, cytoplasmic Kit ELISA, Malate dehydrogenase 1 Kit ELISA, MDH1 Kit ELISA, ME2 Kit ELISA, Mdh1 Kit ELISA, mdh1.S Kit ELISA, LOC100280767 Kit ELISA

Sujet:

Gene Name: Malate Dehydrogenase 1

Gene Aliases: MDHA, Malate Dehydrogenase 1,NAD(Soluble), Cytosolic malate dehydrogenase, Diiodophenylpyruvate reductase