MASP1 Kit ELISA

N° du produit ABIN5656635

Détail du produit MASP1 Kit ELISA

Antigène: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 93.75 pg/mL - 6000 pg/mL

Seuil minimal de détection: 93.75 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mannose Associated Serine Protease 1 (MASP1). No significant cross-reactivity or interference between Mannose Associated Serine Protease 1 (MASP1) and analogues was observed.

Sensibilité: 38.62 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Associated Serine Protease 1 (MASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Associated Serine Protease 1 (MASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Associated Serine Protease 1 (MASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Associated Serine Protease 1 (MASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Associated Serine Protease 1 (MASP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Associated Serine Protease 1 (MASP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Autre désignation: Mannose Associated Serine Protease 1 (MASP1 Produits)

Synonymes: AW048060 Kit ELISA, CCPII Kit ELISA, Crarf Kit ELISA, Masp1/3 Kit ELISA, 3MC1 Kit ELISA, CRARF Kit ELISA, CRARF1 Kit ELISA, MAP1 Kit ELISA, MASP Kit ELISA, MASP3 Kit ELISA, MAp44 Kit ELISA, PRSS5 Kit ELISA, RaRF Kit ELISA, mannan-binding lectin serine peptidase 1 Kit ELISA, mannan binding lectin serine peptidase 1 Kit ELISA, Masp1 Kit ELISA, MASP1 Kit ELISA

Sujet:

Gene Name: Mannose Associated Serine Protease 1

Gene Aliases: CRARF, PRSS5, Mannan-Binding Lectin Serine Peptidase 1, Ra-reactive factor serine protease p100, Complement-activating component of Ra-reactive factor, Serine protease 5

ID gène: 5648

UniProt: P48740

Pathways: Système du Complément