MBL2 Kit ELISA

N° du produit ABIN5656640

Détail du produit MBL2 Kit ELISA

Antigène: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Binding Lectin (MBL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannose Binding Lectin (MBL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Binding Lectin (MBL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Binding Lectin (MBL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannose Binding Lectin (MBL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MBL2 (Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2))

Autre désignation: Mannose Binding Lectin (MBL2 Produits)

Synonymes: COLEC1 Kit ELISA, HSMBPC Kit ELISA, MBL Kit ELISA, MBL2D Kit ELISA, MBP Kit ELISA, MBP-C Kit ELISA, MBP1 Kit ELISA, MBPD Kit ELISA, pMBP-27 Kit ELISA, mbl2 Kit ELISA, MBL1 Kit ELISA, cMBl Kit ELISA, collectin Kit ELISA, Ab2-001 Kit ELISA, Ab2-011 Kit ELISA, L-MBP Kit ELISA, MBL-C Kit ELISA, COLEC2 Kit ELISA, mbl Kit ELISA, etID42583.2 Kit ELISA, fb68b07 Kit ELISA, hbl3 Kit ELISA, wu:fb68b07 Kit ELISA, zgc:109836 Kit ELISA, mannose binding lectin 2 Kit ELISA, Mannose-binding protein C Kit ELISA, mannose-binding lectin (protein C) 2, soluble Kit ELISA, mannose-binding lectin (protein C) 2 Kit ELISA, mannose-binding lectin family member 3, pseudogene Kit ELISA, MBL2 Kit ELISA, mbl2 Kit ELISA, Mbl2 Kit ELISA, MBL3P Kit ELISA

Sujet:

Gene Name: Mannose Binding Lectin

Gene Aliases: MBL2, COLEC1, HSMBPC, MBP1, MBP, Collectin-1, Mannose-binding protein C, Mannan Binding Protein, Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)

ID gène: 4153

UniProt: P11226

Pathways: Système du Complément, Positive Regulation of Immune Effector Process