MAN1A1 Kit ELISA

N° du produit ABIN5656653

Détail du produit MAN1A1 Kit ELISA

Antigène: MAN1A1 (Mannosidase, Alpha, Class 1A, Member 1 (MAN1A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mannosidase Alpha Class 1A Member 1 (MAN1A1). No significant cross-reactivity or interference between Mannosidase Alpha Class 1A Member 1 (MAN1A1) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannosidase Alpha Class 1A Member 1 (MAN1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mannosidase Alpha Class 1A Member 1 (MAN1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannosidase Alpha Class 1A Member 1 (MAN1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannosidase Alpha Class 1A Member 1 (MAN1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mannosidase Alpha Class 1A Member 1 (MAN1A1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mannosidase Alpha Class 1A Member 1 (MAN1A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MAN1A1 (Mannosidase, Alpha, Class 1A, Member 1 (MAN1A1))

Autre désignation: Mannosidase Alpha Class 1A Member 1 (MAN1A1 Produits)

Synonymes: HUMM3 Kit ELISA, HUMM9 Kit ELISA, MAN9 Kit ELISA, Aa2-166 Kit ELISA, Man1a Kit ELISA, MAN1A Kit ELISA, si:dkey-164k19.1 Kit ELISA, si:dkeyp-6c7.1 Kit ELISA, Afu6g06790 Kit ELISA, AO090003000057 Kit ELISA, mannosidase alpha class 1A member 1 Kit ELISA, mannosidase, alpha, class 1A, member 1 Kit ELISA, mannosyl-oligosaccharide alpha-1,2-mannosidase Kit ELISA, Mannosyl-oligosaccharide alpha-1,2-mannosidase Kit ELISA, mannosyl-oligosaccharide alpha-1,2-mannosidase IA Kit ELISA, hypothetical protein Kit ELISA, MAN1A1 Kit ELISA, Man1a1 Kit ELISA, man1a1 Kit ELISA, AFUA_6G06790 Kit ELISA, TVAG_440710 Kit ELISA, LOC5578387 Kit ELISA, AOR_1_100154 Kit ELISA, CC1G_01001 Kit ELISA, PHATRDRAFT_52346 Kit ELISA, TSTA_047320 Kit ELISA, PGTG_11423 Kit ELISA

Sujet:

Gene Name: Mannosidase Alpha Class 1A Member 1

Gene Aliases: HUMM3, HUMM9, MAN9, Mannosyl-Oligosaccharide 1,2-Alpha-Mannosidase IA, Mannosidase alpha class 1A member 1, Processing alpha-1,2-mannosidase IA

ID gène: 4121

UniProt: P33908