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MCAM Kit ELISA

MCAM Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5656811
  • Antigène Voir toutes MCAM Kits ELISA
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    Reactivité
    • 6
    • 3
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Melanoma Cell Adhesion Molecule (MCAM). No significant cross-reactivity or interference between Melanoma Cell Adhesion Molecule (MCAM) and analogues was observed.
    Sensibilité
    0.121 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Cell Adhesion Molecule (MCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Cell Adhesion Molecule (MCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MCAM Kits ELISA
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    Autre désignation
    Melanoma Cell Adhesion Molecule (MCAM Produits)
    Synonymes
    CD146 Kit ELISA, MUC18 Kit ELISA, Muc18 Kit ELISA, 1-gicerin Kit ELISA, AV025631 Kit ELISA, CD149 Kit ELISA, s-endo Kit ELISA, s-gicerin Kit ELISA, cd146 Kit ELISA, gicerin Kit ELISA, id:ibd3512 Kit ELISA, mcam Kit ELISA, MCAM Kit ELISA, melanoma cell adhesion molecule Kit ELISA, melanoma cell adhesion molecule b Kit ELISA, MCAM Kit ELISA, Mcam Kit ELISA, mcamb Kit ELISA
    Sujet

    Gene Name: Melanoma Cell Adhesion Molecule

    Gene Aliases: CD146, MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, S-endo 1 endothelial-associated antigen

    ID gène
    4162
    UniProt
    P43121
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