MCAM Kit CLIA

N° du produit ABIN5656812

Détail du produit MCAM Kit CLIA

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 40 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Melanoma Cell Adhesion Molecule (MCAM). No significant cross-reactivity or interference between Melanoma Cell Adhesion Molecule (MCAM) and analogues was observed.

Sensibilité: 0.03 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Melanoma Cell Adhesion Molecule (MCAM) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Autre désignation: Melanoma Cell Adhesion Molecule (MCAM Produits)

Synonymes: CD146 Kit CLIA, MUC18 Kit CLIA, Muc18 Kit CLIA, 1-gicerin Kit CLIA, AV025631 Kit CLIA, CD149 Kit CLIA, s-endo Kit CLIA, s-gicerin Kit CLIA, cd146 Kit CLIA, gicerin Kit CLIA, id:ibd3512 Kit CLIA, mcam Kit CLIA, MCAM Kit CLIA, melanoma cell adhesion molecule Kit CLIA, melanoma cell adhesion molecule b Kit CLIA, MCAM Kit CLIA, Mcam Kit CLIA, mcamb Kit CLIA

Sujet:

Gene Name: Melanoma Cell Adhesion Molecule

Gene Aliases: CD146, MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, S-endo 1 endothelial-associated antigen

ID gène: 84004

UniProt: Q8R2Y2