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MEP1A Kit ELISA

MEP1A Reactivité: Humain Colorimetric Competition ELISA 49.38 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5656828
  • Antigène Voir toutes MEP1A Kits ELISA
    MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
    Reactivité
    • 5
    • 4
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    49.38 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    49.38 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Meprin A Alpha (MEP1a). No significant cross-reactivity or interference between Meprin A Alpha (MEP1a) and analogues was observed.
    Sensibilité
    19.3 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Meprin A Alpha (MEP1a) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Meprin A Alpha (MEP1a) and unlabeled Meprin A Alpha (MEP1a) (Standards or samples) with the pre-coated antibody specific to Meprin A Alpha (MEP1a). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Meprin A Alpha (MEP1a) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Meprin A Alpha (MEP1a) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meprin A Alpha (MEP1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MEP1A Kits ELISA
    MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
    Autre désignation
    Meprin A Alpha (MEP1A Produits)
    Synonymes
    AI098089 Kit ELISA, AW107200 Kit ELISA, Mep-1 Kit ELISA, Mep-1a Kit ELISA, Mep1 Kit ELISA, PPHA Kit ELISA, meprin A subunit alpha Kit ELISA, meprin 1 alpha Kit ELISA, Mep1a Kit ELISA, MEP1A Kit ELISA
    Sujet

    Gene Name: Meprin A Alpha

    Gene Aliases: PPHA, PABA Peptide Hydrolase, Endopeptidase-2, N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha, PABA Peptide Hydrolase

    ID gène
    4224
    UniProt
    Q16819
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