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METRN Kit ELISA

METRN Reactivité: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5656855
  • Antigène Voir toutes METRN Kits ELISA
    METRN (Meteorin, Glial Cell Differentiation Regulator (METRN))
    Reactivité
    • 5
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Meteorin (METRN). No significant cross-reactivity or interference between Meteorin (METRN) and analogues was observed.
    Sensibilité
    0.054 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meteorin (METRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Meteorin (METRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Meteorin (METRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Meteorin (METRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Meteorin (METRN) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Meteorin (METRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes METRN Kits ELISA
    METRN (Meteorin, Glial Cell Differentiation Regulator (METRN))
    Autre désignation
    Meteorin (METRN Produits)
    Synonymes
    mtrn Kit ELISA, zgc:92132 Kit ELISA, wu:fb81a08 Kit ELISA, METRN Kit ELISA, C16orf23 Kit ELISA, c380A1.2 Kit ELISA, Hyrac Kit ELISA, RGD1306222 Kit ELISA, 1810034B16Rik Kit ELISA, meteorin, glial cell differentiation regulator Kit ELISA, meteorin Kit ELISA, METRN Kit ELISA, metrn Kit ELISA, Metrn Kit ELISA
    Sujet

    Gene Name: Meteorin

    Gene Aliases: C16orf23, RJD6, Glial Cell Differentiation Regulator

    ID gène
    287151
    UniProt
    Q5Q0T9
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