MAT2A Kit ELISA

N° du produit ABIN5656862

Détail du produit MAT2A Kit ELISA

Antigène: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Methionine Adenosyltransferase II Alpha (MAT2a). No significant cross-reactivity or interference between Methionine Adenosyltransferase II Alpha (MAT2a) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase II Alpha (MAT2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine Adenosyltransferase II Alpha (MAT2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionine Adenosyltransferase II Alpha (MAT2a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionine Adenosyltransferase II Alpha (MAT2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Autre désignation: Methionine Adenosyltransferase II Alpha (MAT2A Produits)

Synonymes: MATA2 Kit ELISA, MATII Kit ELISA, SAMS2 Kit ELISA, Sams2 Kit ELISA, D630045P18Rik Kit ELISA, fd12a12 Kit ELISA, mat2a Kit ELISA, si:ch73-340n8.1 Kit ELISA, wu:fb95e01 Kit ELISA, wu:fd12a12 Kit ELISA, m(2)21ab Kit ELISA, MGC76253 Kit ELISA, MGC79598 Kit ELISA, zgc:110847 Kit ELISA, methionine adenosyltransferase 2A Kit ELISA, methionine adenosyltransferase II, alpha Kit ELISA, methionine adenosyltransferase II, alpha a Kit ELISA, methionine adenosyltransferase II, alpha L homeolog Kit ELISA, methionine adenosyltransferase II, alpha b Kit ELISA, MAT2A Kit ELISA, Mat2a Kit ELISA, mat2aa Kit ELISA, mat2a.L Kit ELISA, mat2a Kit ELISA, mat2ab Kit ELISA

Sujet:

Gene Name: Methionine Adenosyltransferase II Alpha

Gene Aliases: SAMS2, MATA2, AMS2, MATII, S-adenosylmethionine synthase isoform type-2

ID gène: 4144

UniProt: P31153

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process