MECP2 Kit ELISA

N° du produit ABIN5656868

Détail du produit MECP2 Kit ELISA

Antigène: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Methyl CpG Binding Protein 2 (MECP2). No significant cross-reactivity or interference between Methyl CpG Binding Protein 2 (MECP2) and analogues was observed.

Sensibilité: 0.127 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methyl CpG Binding Protein 2 (MECP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methyl CpG Binding Protein 2 (MECP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methyl CpG Binding Protein 2 (MECP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methyl CpG Binding Protein 2 (MECP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methyl CpG Binding Protein 2 (MECP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methyl CpG Binding Protein 2 (MECP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Autre désignation: Methyl CpG Binding Protein 2 (MECP2 Produits)

Synonymes: AUTSX3 Kit ELISA, MRX16 Kit ELISA, MRX79 Kit ELISA, MRXS13 Kit ELISA, MRXSL Kit ELISA, PPMX Kit ELISA, RS Kit ELISA, RTS Kit ELISA, RTT Kit ELISA, 1500041B07Rik Kit ELISA, BB130002 Kit ELISA, D630021H01Rik Kit ELISA, Mbd5 Kit ELISA, WBP10 Kit ELISA, wu:fk96a04 Kit ELISA, zgc:111857 Kit ELISA, methyl-CpG binding protein 2 Kit ELISA, methyl CpG binding protein 2 Kit ELISA, methyl-CpG binding protein 2 S homeolog Kit ELISA, MECP2 Kit ELISA, Mecp2 Kit ELISA, mecp2 Kit ELISA, mecp2.S Kit ELISA

Sujet:

Gene Name: Methyl CpG Binding Protein 2

Gene Aliases: RTS, AUTSX3, MRX16, MRX79, PPMX, RTT, Rett Syndrome, Mental Retardation,X-linked 16

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane