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MAP1LC3A Kit ELISA

MAP1LC3A Reactivité: Humain Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5656887
  • Antigène Voir toutes MAP1LC3A Kits ELISA
    MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
    Reactivité
    • 3
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56 ng/mL - 100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). No significant cross-reactivity or interference between Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) and analogues was observed.
    Sensibilité
    0.59 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MAP1LC3A Kits ELISA
    MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
    Autre désignation
    Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3A Produits)
    Synonymes
    ATG8E Kit ELISA, LC3 Kit ELISA, LC3A Kit ELISA, MAP1ALC3 Kit ELISA, MAP1BLC3 Kit ELISA, 1010001H21Rik Kit ELISA, 4922501H04Rik Kit ELISA, LC3a Kit ELISA, MGC69006 Kit ELISA, zgc:77094 Kit ELISA, MGC89867 Kit ELISA, MAP1LC3A Kit ELISA, map1lc3a Kit ELISA, microtubule associated protein 1 light chain 3 alpha Kit ELISA, microtubule-associated protein 1 light chain 3 alpha Kit ELISA, microtubule associated protein 1 light chain 3 alpha S homeolog Kit ELISA, MAP1LC3A Kit ELISA, Map1lc3a Kit ELISA, map1lc3a.S Kit ELISA, map1lc3a Kit ELISA
    Sujet

    Gene Name: Microtubule Associated Protein 1 Light Chain 3 Alpha

    Gene Aliases: MAP1ALC3, MAP1BLC3, LC3, LC3A, ATG8E, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1

    ID gène
    84557
    UniProt
    Q9H492
    Pathways
    Autophagy
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