SLC25A37 Kit ELISA

N° du produit ABIN5656919

Détail du produit SLC25A37 Kit ELISA

Antigène: SLC25A37 (Solute Carrier Family 25, Member 37 (SLC25A37))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mitoferrin (MFRN). No significant cross-reactivity or interference between Mitoferrin (MFRN) and analogues was observed.

Sensibilité: 0.053 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitoferrin (MFRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitoferrin (MFRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitoferrin (MFRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitoferrin (MFRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitoferrin (MFRN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitoferrin (MFRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SLC25A37 (Solute Carrier Family 25, Member 37 (SLC25A37))

Autre désignation: Mitoferrin (SLC25A37 Produits)

Synonymes: MFRN Kit ELISA, MFRN1 Kit ELISA, MSC Kit ELISA, MSCP Kit ELISA, PRO1278 Kit ELISA, PRO1584 Kit ELISA, PRO2217 Kit ELISA, 1700020E22Rik Kit ELISA, 4930513O14Rik Kit ELISA, 4930526G11Rik Kit ELISA, AI848481 Kit ELISA, C330015G08Rik Kit ELISA, Mfrn Kit ELISA, Mfrn1 Kit ELISA, Mscp Kit ELISA, frascati Kit ELISA, mitoferrin Kit ELISA, RGD1359361 Kit ELISA, fc73c03 Kit ELISA, wu:fc73c03 Kit ELISA, SLC25A37 Kit ELISA, solute carrier family 25 member 37 Kit ELISA, solute carrier family 25, member 37 Kit ELISA, solute carrier family 25 (mitochondrial iron transporter), member 37 Kit ELISA, solute carrier family 25 (mitochondrial iron transporter), member 37 S homeolog Kit ELISA, SLC25A37 Kit ELISA, Slc25a37 Kit ELISA, slc25a37 Kit ELISA, slc25a37.S Kit ELISA

Sujet:

Gene Name: Mitoferrin

Gene Aliases: SLC25A37, MSC, HT015, MSCP, Solute Carrier Family 25 Member 37, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein

ID gène: 51312

UniProt: Q9NYZ2

Pathways: Transition Metal Ion Homeostasis