MEK1 Kit ELISA

N° du produit ABIN5656926

Détail du produit MEK1 Kit ELISA

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). No significant cross-reactivity or interference between Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase Kinase 1 (MAP2K1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Autre désignation: Mitogen Activated Protein Kinase Kinase 1 (MAP2K1 Produits)

Synonymes: Mek1 Kit ELISA, CFC3 Kit ELISA, MAPKK1 Kit ELISA, MEK1 Kit ELISA, MKK1 Kit ELISA, PRKMK1 Kit ELISA, MEKK1 Kit ELISA, Prkmk1 Kit ELISA, mek-2 Kit ELISA, si:ch211-242m18.2 Kit ELISA, wu:fj56a12 Kit ELISA, wu:fj61b01 Kit ELISA, zgc:56557 Kit ELISA, ATMEK1 Kit ELISA, F20B18.180 Kit ELISA, F20B18_180 Kit ELISA, MAP kinase/ ERK kinase 1 Kit ELISA, MITOGEN ACTIVATED PROTEIN KINASE KINASE Kit ELISA, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 Kit ELISA, NMAPKK Kit ELISA, MEK Kit ELISA, mitogen activated protein kinase kinase 1 Kit ELISA, mitogen-activated protein kinase kinase 1 Kit ELISA, mitogen-activated protein kinase kinase 1 L homeolog Kit ELISA, MAP kinase/ ERK kinase 1 Kit ELISA, Map2k1 Kit ELISA, MAP2K1 Kit ELISA, map2k1.L Kit ELISA, map2k1 Kit ELISA, MEK1 Kit ELISA

Sujet:

Gene Name: Mitogen Activated Protein Kinase Kinase 1

Gene Aliases: MAPKK1, MEK1, MKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1

ID gène: 5604

UniProt: Q02750

Pathways: Signalisation MAPK, Signalisation RTK, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling