MLH1 Kit ELISA

N° du produit ABIN5657021

Détail du produit MLH1 Kit ELISA

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of MutL Homolog 1 (MLH1). No significant cross-reactivity or interference between MutL Homolog 1 (MLH1) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MutL Homolog 1 (MLH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MutL Homolog 1 (MLH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MutL Homolog 1 (MLH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MutL Homolog 1 (MLH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MutL Homolog 1 (MLH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MutL Homolog 1 (MLH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MutL Homolog 1 (MLH1 Produits)

Synonymes: CG11482 Kit ELISA, Dmel\\CG11482 Kit ELISA, dmlh-1 Kit ELISA, dmlh1 Kit ELISA, zgc:66301 Kit ELISA, MLH1 Kit ELISA, LOC100232198 Kit ELISA, 1110035C23Rik Kit ELISA, AI317206 Kit ELISA, AI325952 Kit ELISA, AI561766 Kit ELISA, COCA2 Kit ELISA, FCC2 Kit ELISA, HNPCC Kit ELISA, HNPCC2 Kit ELISA, hMLH1 Kit ELISA, mutL homolog 1 Kit ELISA, CG11482 gene product from transcript CG11482-RB Kit ELISA, mutL homolog 1 S homeolog Kit ELISA, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) Kit ELISA, DNA mismatch repair protein Mlh1 Kit ELISA, MLH1 Kit ELISA, Mlh1 Kit ELISA, mlh1.S Kit ELISA, mlh1 Kit ELISA, LOC588545 Kit ELISA

Sujet:

Gene Name: MutL Homolog 1

Gene Aliases: COCA2, FCC2, HNPCC, HNPCC2, hMLH1, DNA mismatch repair protein Mlh1

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response