MAX Kit ELISA

N° du produit ABIN5657025

Détail du produit MAX Kit ELISA

Antigène: MAX (MYC Associated Factor X (MAX))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of MYC Associated Factor X (MAX). No significant cross-reactivity or interference between MYC Associated Factor X (MAX) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYC Associated Factor X (MAX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYC Associated Factor X (MAX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYC Associated Factor X (MAX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYC Associated Factor X (MAX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MYC Associated Factor X (MAX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MYC Associated Factor X (MAX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MAX (MYC Associated Factor X (MAX))

Autre désignation: MYC Associated Factor X (MAX Produits)

Synonymes: CG9648 Kit ELISA, Dmel\\CG9648 Kit ELISA, MAX Kit ELISA, bHLHd9 Kit ELISA, dMax Kit ELISA, dmax Kit ELISA, max Kit ELISA, bhlhd4 Kit ELISA, bhlhd5 Kit ELISA, bhlhd6 Kit ELISA, bhlhd7 Kit ELISA, bhlhd8 Kit ELISA, xmax2 Kit ELISA, xmax4 Kit ELISA, xmax Kit ELISA, AA960152 Kit ELISA, AI875693 Kit ELISA, bHLHd4 Kit ELISA, bHLHd5 Kit ELISA, bHLHd6 Kit ELISA, bHLHd7 Kit ELISA, bHLHd8 Kit ELISA, fa92b04 Kit ELISA, fb52a04 Kit ELISA, fb95h11 Kit ELISA, wu:fa92b04 Kit ELISA, wu:fb52a04 Kit ELISA, wu:fb95h11 Kit ELISA, zmax Kit ELISA, CG9648 gene product from transcript CG9648-RD Kit ELISA, MYC associated factor X L homeolog Kit ELISA, MYC associated factor X Kit ELISA, Max protein Kit ELISA, MYC associated factor X S homeolog Kit ELISA, myc associated factor X Kit ELISA, Max Kit ELISA, max.L Kit ELISA, MAX Kit ELISA, max Kit ELISA, max.S Kit ELISA

Sujet:

Gene Name: MYC Associated Factor X

Gene Aliases: BHLHD4, Class D basic helix-loop-helix protein 4

ID gène: 4149

UniProt: P61244

Pathways: Mitotic G1-G1/S Phases