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MCL-1 Kit ELISA

MCL1 Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5657044
  • Antigène Voir toutes MCL-1 (MCL1) Kits ELISA
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    Reactivité
    • 6
    • 2
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). No significant cross-reactivity or interference between Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) and analogues was observed.
    Sensibilité
    0.063 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MCL-1 (MCL1) Kits ELISA
    MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))
    Autre désignation
    Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1 Produits)
    Synonymes
    BCL2L3 Kit ELISA, EAT Kit ELISA, MCL1-ES Kit ELISA, MCL1L Kit ELISA, MCL1S Kit ELISA, Mcl-1 Kit ELISA, TM Kit ELISA, bcl2-L-3 Kit ELISA, mcl1/EAT Kit ELISA, AW556805 Kit ELISA, mcl-1 Kit ELISA, fb72f02 Kit ELISA, wu:fb70a08 Kit ELISA, wu:fb72f02 Kit ELISA, zfMLP1 Kit ELISA, zfMcl-1a Kit ELISA, zgc:109835 Kit ELISA, zgc:109763 Kit ELISA, E3 Kit ELISA, E3-2 Kit ELISA, RNE3 Kit ELISA, RNU107 Kit ELISA, SNORA63A Kit ELISA, elF-4AII Kit ELISA, MCL1, BCL2 family apoptosis regulator Kit ELISA, myeloid cell leukemia sequence 1 Kit ELISA, MCL1, BCL2 family apoptosis regulator a Kit ELISA, MCL1, BCL2 family apoptosis regulator b Kit ELISA, small nucleolar RNA, H/ACA box 63 Kit ELISA, MCL1 Kit ELISA, Mcl1 Kit ELISA, mcl1a Kit ELISA, mcl1b Kit ELISA, SNORA63 Kit ELISA
    Sujet

    Gene Name: Myeloid Cell Leukemia Sequence 1, Bcl2 Related

    Gene Aliases: EAT, MCL1L, MCL1S, BCL2L3, TM, Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT

    ID gène
    17210
    UniProt
    P97287
    Pathways
    Signalisation MAPK
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