MIOX Kit ELISA

N° du produit ABIN5657066

Détail du produit MIOX Kit ELISA

Antigène: MIOX (Myo-Inositol Oxygenase (MIOX))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Myo Inositol Oxygenase (MIOX). No significant cross-reactivity or interference between Myo Inositol Oxygenase (MIOX) and analogues was observed.

Sensibilité: 0.069 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myo Inositol Oxygenase (MIOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myo Inositol Oxygenase (MIOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myo Inositol Oxygenase (MIOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myo Inositol Oxygenase (MIOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myo Inositol Oxygenase (MIOX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myo Inositol Oxygenase (MIOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MIOX (Myo-Inositol Oxygenase (MIOX))

Autre désignation: Myo Inositol Oxygenase (MIOX Produits)

Synonymes: ALDRL6 Kit ELISA, 0610009I10Rik Kit ELISA, AI314022 Kit ELISA, Aldrl6 Kit ELISA, C85427 Kit ELISA, RSOR Kit ELISA, MGC82300 Kit ELISA, MGC86377 Kit ELISA, aldrl6 Kit ELISA, zgc:114168 Kit ELISA, DDBDRAFT_0188751 Kit ELISA, DDBDRAFT_0235367 Kit ELISA, DDB_0188751 Kit ELISA, DDB_0235367 Kit ELISA, MI oxygenase Kit ELISA, myo-inositol oxygenase Kit ELISA, myo-inositol oxygenase L homeolog Kit ELISA, inositol oxygenase 1 Kit ELISA, inositol oxygenase Kit ELISA, Myo-inositol oxygenase Kit ELISA, MIOX Kit ELISA, Miox Kit ELISA, miox.L Kit ELISA, miox Kit ELISA, MGG_09462 Kit ELISA, CNF03950 Kit ELISA, CC1G_10713 Kit ELISA, SCHCODRAFT_258535 Kit ELISA, CGB_F1290W Kit ELISA, mioX Kit ELISA, LOC100101177 Kit ELISA, LOC107131132 Kit ELISA

Sujet:

Gene Name: Myo Inositol Oxygenase

Gene Aliases: ALDRL6, KSP32, RSOR, Kidney-Specific Protein 32, Aldehyde Reductase(Aldose Reductase)Like 6, Renal-specific oxidoreductase, Myo-inositol oxygenase

Pathways: Inositol Metabolic Process