MYH3 Kit ELISA

N° du produit ABIN5657091

Détail du produit MYH3 Kit ELISA

Antigène: MYH3 (Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). No significant cross-reactivity or interference between Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MYH3 (Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3))

Autre désignation: Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3 Produits)

Synonymes: HEMHC Kit ELISA, MYHC-EMB Kit ELISA, MYHSE1 Kit ELISA, SMHCE Kit ELISA, Myhse Kit ELISA, RNMHCG Kit ELISA, MyHC-emb Kit ELISA, Myhs-e Kit ELISA, MHC A Kit ELISA, MYH1B Kit ELISA, MYH3 Kit ELISA, Myosin-1B Kit ELISA, Myosin-3 Kit ELISA, myosin heavy chain 3 Kit ELISA, myosin, heavy polypeptide 3, skeletal muscle, embryonic Kit ELISA, Myosin-3 Kit ELISA, myosin, heavy chain 1D, skeletal muscle Kit ELISA, myosin, heavy chain 3, skeletal muscle, embryonic Kit ELISA, MYH3 Kit ELISA, Myh3 Kit ELISA, myo-3 Kit ELISA, MYH1D Kit ELISA

Sujet:

Gene Name: Myosin Heavy Chain 3, Skeletal Muscle, Embryonic

Gene Aliases: HEMHC, MYHC-EMB, MYHSE1, SMHCE, Myosin,Skeletal,Heavy chain,Embryonic 1, Myosin heavy chain, fast skeletal muscle, embryonic

ID gène: 4621

UniProt: P11055