Myosin 9 Kit ELISA

N° du produit ABIN5657102

Détail du produit Myosin 9 Kit ELISA

Antigène: Myosin 9 (MYH9)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 9, Non Muscle (MYH9). No significant cross-reactivity or interference between Myosin Heavy Chain 9, Non Muscle (MYH9) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 9, Non Muscle (MYH9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 9, Non Muscle (MYH9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 9, Non Muscle (MYH9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 9, Non Muscle (MYH9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 9, Non Muscle (MYH9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 9, Non Muscle (MYH9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Myosin 9 (MYH9)

Autre désignation: Myosin Heavy Chain 9, Non Muscle (MYH9 Produits)

Synonymes: BDPLT6 Kit ELISA, DFNA17 Kit ELISA, EPSTS Kit ELISA, FTNS Kit ELISA, MHA Kit ELISA, NMHC-II-A Kit ELISA, NMMHC-IIA Kit ELISA, NMMHCA Kit ELISA, C80049 Kit ELISA, D0Jmb2 Kit ELISA, E030044M24Rik Kit ELISA, Fltn Kit ELISA, Myhn-1 Kit ELISA, Myhn1 Kit ELISA, NMHC II-A Kit ELISA, NMHCIIA Kit ELISA, NMMHC II-a Kit ELISA, NMMHC-A Kit ELISA, TU72.6 Kit ELISA, fi22c04 Kit ELISA, myh9 Kit ELISA, myh9l2 Kit ELISA, wu:fi22c04 Kit ELISA, wu:fj85e11 Kit ELISA, zgc:162029 Kit ELISA, zgc:66164 Kit ELISA, NMMHC Kit ELISA, myosin Kit ELISA, non-muscle Kit ELISA, nonmuscle Kit ELISA, KLG/PTK7 Kit ELISA, dfna17 Kit ELISA, epsts Kit ELISA, ftns Kit ELISA, nmhc-ii-a Kit ELISA, nmmhca Kit ELISA, myosin heavy chain 9 Kit ELISA, myosin, heavy polypeptide 9, non-muscle Kit ELISA, myosin, heavy chain 9a, non-muscle Kit ELISA, myosin, heavy chain 9, non-muscle Kit ELISA, myosin, heavy chain 9, non-muscle L homeolog Kit ELISA, MYH9 Kit ELISA, Myh9 Kit ELISA, myh9a Kit ELISA, myh9.L Kit ELISA

Sujet:

Gene Name: Myosin Heavy Chain 9, Non Muscle

Gene Aliases: DFNA17, EPSTS, FTNS, MHA, NMHC-II-A, NMMHCA, Nonmuscle Myosin Heavy Chain II-A, Cellular myosin heavy chain, type A, Myosin heavy chain, non-muscle IIa

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Integrin Complex