MYL1 Kit ELISA

N° du produit ABIN5657103

Détail du produit MYL1 Kit ELISA

Antigène: MYL1 (Myosin, Light Chain 1, Alkali, Skeletal, Fast (MYL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1). No significant cross-reactivity or interference between Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MYL1 (Myosin, Light Chain 1, Alkali, Skeletal, Fast (MYL1))

Autre désignation: Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1 Produits)

Synonymes: MLC1F Kit ELISA, MLC3F Kit ELISA, alkali Kit ELISA, fast Kit ELISA, myosin Kit ELISA, skeletal Kit ELISA, AI325107 Kit ELISA, MLC1f Kit ELISA, MLC3f Kit ELISA, Mylf Kit ELISA, MLC3-f Kit ELISA, MLCf Kit ELISA, Mcl3 Kit ELISA, Mlc1 Kit ELISA, Mlc3 Kit ELISA, fj53a11 Kit ELISA, wu:fj53a11 Kit ELISA, zgc:65785 Kit ELISA, zgc:77231 Kit ELISA, MLC1f/3f Kit ELISA, XMLC1f Kit ELISA, XMLC3f Kit ELISA, myl1 Kit ELISA, myosin light chain 1 Kit ELISA, myosin, light chain 1, alkali; skeletal, fast Kit ELISA, myosin, light polypeptide 1 Kit ELISA, myosin, light chain 1 Kit ELISA, Myosin regulatory light chain 1 Kit ELISA, myosin light chain 1 L homeolog Kit ELISA, MYL1 Kit ELISA, Myl1 Kit ELISA, mlc-1 Kit ELISA, myl1 Kit ELISA, myl1.L Kit ELISA

Sujet:

Gene Name: Myosin Light Chain 1, Alkali, Fast Skeletal

Gene Aliases: MLC1, MLC1F, MLC3F, Myosin light chain 1/3, skeletal muscle isoform, Myosin light chain A1/A2

ID gène: 4632

UniProt: P05976