MYLK Kit ELISA

N° du produit ABIN5657107

Détail du produit MYLK Kit ELISA

Antigène: MYLK (Myosin Light Chain Kinase (MYLK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain Kinase (MYLK). No significant cross-reactivity or interference between Myosin Light Chain Kinase (MYLK) and analogues was observed.

Sensibilité: 0.053 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain Kinase (MYLK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain Kinase (MYLK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain Kinase (MYLK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain Kinase (MYLK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain Kinase (MYLK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain Kinase (MYLK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MYLK (Myosin Light Chain Kinase (MYLK))

Autre désignation: Myosin Light Chain Kinase (MYLK Produits)

Synonymes: AAT7 Kit ELISA, KRP Kit ELISA, MLCK Kit ELISA, MLCK1 Kit ELISA, MLCK108 Kit ELISA, MLCK210 Kit ELISA, MSTP083 Kit ELISA, MYLK1 Kit ELISA, smMLCK Kit ELISA, 9530072E15Rik Kit ELISA, A930019C19Rik Kit ELISA, AW489456 Kit ELISA, Mlck Kit ELISA, SMMLCK Kit ELISA, mlck Kit ELISA, fj60a06 Kit ELISA, mlck1a Kit ELISA, mylk Kit ELISA, wu:fj60a06 Kit ELISA, zmlck1 Kit ELISA, myosin light chain kinase Kit ELISA, myosin, light polypeptide kinase Kit ELISA, myosin, light chain kinase a Kit ELISA, MYLK Kit ELISA, Mylk Kit ELISA, mylka Kit ELISA

Sujet:

Gene Name: Myosin Light Chain Kinase

Gene Aliases: KRP, MLCK108, MLCK210, MSTP083, MLCK, s mMLCK, MYLK1, MLCK1, Telokin, Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form, Kinase-related protein

ID gène: 4638

UniProt: Q15746