MPG Kit ELISA

N° du produit ABIN5657149

Détail du produit MPG Kit ELISA

Antigène: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of N-Methylpurine DNA Glycosylase (MPG). No significant cross-reactivity or interference between N-Methylpurine DNA Glycosylase (MPG) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Methylpurine DNA Glycosylase (MPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Methylpurine DNA Glycosylase (MPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Methylpurine DNA Glycosylase (MPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Methylpurine DNA Glycosylase (MPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Methylpurine DNA Glycosylase (MPG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Methylpurine DNA Glycosylase (MPG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Autre désignation: N-Methylpurine DNA Glycosylase (MPG Produits)

Synonymes: AAG Kit ELISA, ADPG Kit ELISA, APNG Kit ELISA, CRA36.1 Kit ELISA, MDG Kit ELISA, Mid1 Kit ELISA, PIG11 Kit ELISA, PIG16 Kit ELISA, anpg Kit ELISA, 9830006D05 Kit ELISA, AI326268 Kit ELISA, Aag Kit ELISA, zgc:162984 Kit ELISA, si:xx-187g17.9 Kit ELISA, MPG Kit ELISA, MPGR Kit ELISA, N-methylpurine DNA glycosylase Kit ELISA, N-methylpurine-DNA glycosylase Kit ELISA, si:xx-by187g17.9 Kit ELISA, MPG Kit ELISA, Mpg Kit ELISA, mpg Kit ELISA, si:xx-by187g17.9 Kit ELISA, CpB0526 Kit ELISA, gll2491 Kit ELISA

Sujet:

Gene Name: N-Methylpurine DNA Glycosylase

Gene Aliases: AAG, APNG, ADPG, CRA36.1, MDG, Mid1, PIG11, PIG16, Anpg, DNA-3-Methyladenine Glycosylase, Alkyladenine DNA Glycosylase, 3-alkyladenine DNA glycosylase

ID gène: 4350

UniProt: P29372

Pathways: Réparation de l'ADN