NDRG2 Kit ELISA

N° du produit ABIN5657150

Détail du produit NDRG2 Kit ELISA

Antigène: NDRG2 (NDRG Family Member 2 (NDRG2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of N-myc Downstream Regulated Gene 2 (NDRG2). No significant cross-reactivity or interference between N-myc Downstream Regulated Gene 2 (NDRG2) and analogues was observed.

Sensibilité: 0.141 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-myc Downstream Regulated Gene 2 (NDRG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-myc Downstream Regulated Gene 2 (NDRG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-myc Downstream Regulated Gene 2 (NDRG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-myc Downstream Regulated Gene 2 (NDRG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-myc Downstream Regulated Gene 2 (NDRG2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-myc Downstream Regulated Gene 2 (NDRG2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NDRG2 (NDRG Family Member 2 (NDRG2))

Autre désignation: N-myc Downstream Regulated Gene 2 (NDRG2 Produits)

Synonymes: NDRG2 Kit ELISA, AI182517 Kit ELISA, AU040374 Kit ELISA, Ndr2 Kit ELISA, SYLD Kit ELISA, im:6909381 Kit ELISA, si:dkey-88n24.1 Kit ELISA, zgc:101847 Kit ELISA, NDRG family member 2 Kit ELISA, N-myc downstream regulated gene 2 Kit ELISA, NDRG family member 2 S homeolog Kit ELISA, ndrg2 Kit ELISA, NDRG2 Kit ELISA, Ndrg2 Kit ELISA, ndrg2.S Kit ELISA

Sujet:

Gene Name: N-myc Downstream Regulated Gene 2

Gene Aliases: SYLD, N-myc downstream-regulated gene 2 protein

ID gène: 171114

UniProt: Q8VBU2