Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Neuropeptide FF Kit ELISA

NPFF Reactivité: Souris Colorimetric Competition ELISA 31.2 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5657309
  • Antigène Tous les produits Neuropeptide FF (NPFF)
    Neuropeptide FF (NPFF)
    Reactivité
    • 2
    • 2
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    31.2 pg/mL - 500 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Neuropeptide FF (NPFF). No significant cross-reactivity or interference between Neuropeptide FF (NPFF) and analogues was observed.
    Sensibilité
    13.5 pg/mL
    Featured
    Discover our best selling NPFF Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuropeptide FF (NPFF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuropeptide FF (NPFF) and unlabeled Neuropeptide FF (NPFF) (Standards or samples) with the pre-coated antibody specific to Neuropeptide FF (NPFF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuropeptide FF (NPFF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuropeptide FF (NPFF) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide FF (NPFF) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide FF (NPFF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Tous les produits Neuropeptide FF (NPFF)
    Neuropeptide FF (NPFF)
    Autre désignation
    Neuropeptide FF (NPFF Produits)
    Synonymes
    neuropeptide FF-amide peptide precursor Kit ELISA, npff Kit ELISA
    Sujet

    Gene Name: Neuropeptide FF

    Gene Aliases: FMRFAL, Neuropeptide FF-Amide Peptide Precursor, Pro-FMRFamide-related neuropeptide FF

Vous êtes ici:
Support technique