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NOSIP Kit ELISA

NOSIP Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN5657411
  • Antigène Voir toutes NOSIP Kits ELISA
    NOSIP (Nitric Oxide Synthase Interacting Protein (NOSIP))
    Reactivité
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase Interacting Protein (NOSIP). No significant cross-reactivity or interference between Nitric Oxide Synthase Interacting Protein (NOSIP) and analogues was observed.
    Sensibilité
    0.053 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase Interacting Protein (NOSIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase Interacting Protein (NOSIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase Interacting Protein (NOSIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase Interacting Protein (NOSIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase Interacting Protein (NOSIP) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase Interacting Protein (NOSIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes NOSIP Kits ELISA
    NOSIP (Nitric Oxide Synthase Interacting Protein (NOSIP))
    Autre désignation
    Nitric Oxide Synthase Interacting Protein (NOSIP Produits)
    Synonymes
    nosip Kit ELISA, MGC75989 Kit ELISA, NOSIP Kit ELISA, wu:fb80a11 Kit ELISA, zgc:101669 Kit ELISA, 2310061K06Rik Kit ELISA, CGI-25 Kit ELISA, nitric oxide synthase interacting protein Kit ELISA, nitric oxide synthase interacting protein S homeolog Kit ELISA, nosip Kit ELISA, NOSIP Kit ELISA, Nosip Kit ELISA, LOC100280875 Kit ELISA, nosip.S Kit ELISA
    Sujet

    Gene Name: Nitric Oxide Synthase Interacting Protein

    Gene Aliases: CGI-25, eNOS-interacting protein

    ID gène
    51070
    UniProt
    Q9Y314
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