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NONO Kit ELISA

NONO Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5657428
  • Antigène Voir toutes NONO Kits ELISA
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Non POU Domain Containing Octamer Binding Protein (NONO). No significant cross-reactivity or interference between Non POU Domain Containing Octamer Binding Protein (NONO) and analogues was observed.
    Sensibilité
    0.056 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Non POU Domain Containing Octamer Binding Protein (NONO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Non POU Domain Containing Octamer Binding Protein (NONO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Non POU Domain Containing Octamer Binding Protein (NONO) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Non POU Domain Containing Octamer Binding Protein (NONO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes NONO Kits ELISA
    NONO (Non-POU Domain Containing, Octamer-Binding (NONO))
    Autre désignation
    Non POU Domain Containing Octamer Binding Protein (NONO Produits)
    Synonymes
    NMT55 Kit ELISA, NRB54 Kit ELISA, P54 Kit ELISA, P54NRB Kit ELISA, nmt55 Kit ELISA, nrb54 Kit ELISA, p54nrb Kit ELISA, xp54nrb Kit ELISA, AA407051 Kit ELISA, AV149256 Kit ELISA, nonA Kit ELISA, non-POU domain containing octamer binding Kit ELISA, non-POU domain containing, octamer-binding Kit ELISA, non-POU domain containing, octamer binding L homeolog Kit ELISA, non-POU-domain-containing, octamer binding protein Kit ELISA, NONO Kit ELISA, Nono Kit ELISA, nono.L Kit ELISA
    Sujet

    Gene Name: Non POU Domain Containing Octamer Binding Protein

    Gene Aliases: P54, NMT55, NRB54, P54NRB, Nuclear RNA-Binding Protein 54-kD, 54 kDa nuclear RNA- and DNA-binding protein, DNA-binding p52/p100 complex, 52 kDa subunit

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